Abstract Objective: To analyze the morphofunctional regeneration process of facial nerve injury in the presence of insulin-like growth factor-1 and mesenchymal stem cells. Methods: Fourteen Wistar rats suffered unilateral facial nerve crushing and were randomly divided into two groups. All received insulin-like growth factor-1 inoculation, but only half of the animals received an additional inoculation of mesenchymal stem cells. The animals were followed for 90 days and facial nerve regeneration was analyzed via spontaneous facial motor function tests and immunohistochemistry in the nerve motor nucleus. Results: The group that received the growth factor and stem cells showed a statistically superior mean in vibrissae movements (p<0.01), touch reflex (p = 0.05) and eye closure (p<0.01), in addition to better immunohistochemistry reactivity. There was a statistically significant difference in the mean number of cells in the facial nerve nucleus between the experimental groups (p = 0.025), with the group that received the growth factor and stem cells showing the highest mean. Conclusion: The association between growth factor and stem cells potentiates the morphofunctional regeneration of the facial nerve, occurring faster and more effectively. Level of Evidence: 4, degree of recommendation C.

Abstract Objective: To evaluate the positive predictive value (PPV) of amorphous calcifications and to analyze the imaging variables that could alter the risk of malignancy associated with this finding. Materials and Methods: This was a retrospective study of 138 stereotactically guided percutaneous vacuum-assisted biopsies of amorphous calcifications, performed between January 2012 and December 2017. All of the patients included were referred for radiological follow-up for a minimum of one year (if the histopathology showed a benign lesion) or for surgical treatment (if the histopathology showed malignancy or a lesion of uncertain malignant potential). Results: We found that the PPV of amorphous calcifications was 9.42%. However, most of the malignant amorphous calcifications were in cases of invasive carcinoma or high-grade ductal carcinoma in situ, indicating clinically relevant disease. The relative risk of malignancy associated with amorphous calcifications was 6.15 times higher in patients with a family or personal history of breast or ovarian cancer. Neither being postmenopausal nor having dense breasts was found to be predictive of malignancy in patients with amorphous calcifications. Conclusion: Amorphous calcifications in the breast had a PPV for malignancy of 9.42%, indicating the possibility of placing the finding in subcategory 4a, which requires histopathological analysis. Our finding that the risk of malignancy associated with this subtype of calcifications is up to 6.15 times higher in patients with a family or personal history of breast cancer warrants greater concern regarding the clinical, radiologic, and histopathologic correlations after biopsy.

Resumo Objetivo: Avaliar o valor preditivo positivo (VPP) das calcificações amorfas e possíveis variáveis clínicas e de imagem que possam influenciar no risco de malignidade deste achado de imagem. Materiais e Métodos: Foram revisados, retrospectivamente, 138 resultados de biópsias percutâneas estereotáxicas a vácuo de calcificações amorfas, entre janeiro de 2012 e dezembro de 2017. Todas as pacientes incluídas apresentavam seguimento radiológico mínimo de um ano (histopatológico benigno) ou tratamento cirúrgico (histopatológico maligno). Resultados: O VPP das calcificações amorfas foi de 9,42%. As lesões malignas corresponderam predominantemente a carcinomas invasivos, indicando doença clinicamente relevante. O risco relativo de malignidade das calcificações amorfas foi 6,15 vezes maior em pacientes com história familiar ou pessoal de neoplasia de mama ou ovário. Status pós-menopausa e mamas densas não foram preditores de malignidade nessas pacientes. Conclusão: As calcificações amorfas na mama apresentaram VPP de malignidade de 9,42%, sugerindo possibilidade de classificação do achado na subcategoria 4a, com necessidade de investigação histopatológica. Em pacientes com história familiar ou pessoal de câncer de mama, o risco de malignidade deste subtipo de calcificações pode ser até 6,15 vezes maior, justificando maior preocupação na correlação clínica, radiológica e histopatológica após biópsia.

ABSTRACT The COVID-19 pandemic has imposed a health crisis around the world. Health professionals are frequently exposed to stressors that put them at high risk for the development or progression of disabling mental disorders, including posttraumatic stress disorder (PTSD). To understand how pandemic stressors have affected the mental health of healthcare workers, our group conducted a longitudinal and nationwide survey. We investigated the occurrence of traumatic events related to the COVID-19 pandemic and the association between exposure to these events and PTSD symptomatology. Importantly, we also investigated factors that might increase or decrease the risk for PTSD. Depression symptoms were also investigated. The results of the first wave of the project were published in a series of three articles, each focused on different risk or protective factors. The results showed that female sex, young age, a lack of adequate personal protective equipment, social isolation and a previous history of mental illness were consistent predictors of PTSD symptoms. Healthcare workers who reported high levels of peritraumatic tonic immobility, a defensive response that is involuntary, reflexive, and evoked by an intense and inescapable threat, also exhibited an increase in the probability of being diagnosed with PTSD. On the other hand, professional recognition had a negative relationship with PTSD and depression symptoms, emerging as a significant protective factor for psychological health. The identification of protective and risk factors in these situations is crucial to guide the adoption of long-term measures in work environments that will enhance the psychological health of these professionals.

RESUMO A pandemia de COVID-19 impôs uma crise de saúde no mundo. Profissionais de saúde foram frequentemente expostos a estressores que os colocam em alto risco para o desenvolvimento ou progressão de transtornos mentais incapacitantes, incluindo o transtorno de estresse pós-traumático (TEPT). Para entender como esses estressores afetaram a saúde mental desses profissionais, nosso grupo realizou um estudo longitudinal nacional. Investigamos a ocorrência de eventos traumáticos especificamente relacionados à pandemia e a associação entre a exposição a esses eventos traumáticos e a sintomatologia do TEPT. É importante ressaltar que também investigamos fatores que podem aumentar ou diminuir o risco de TEPT. Sintomas de depressão também foram investigados. Os resultados da primeira onda do projeto foram publicados em uma série de três artigos, cada um focado em diferentes fatores de risco ou proteção. Os resultados mostraram que sexo feminino, idade jovem, falta de equipamento de proteção individual adequado, isolamento social e história prévia de doença mental foram preditores consistentes de sintomas de TEPT. Os profissionais de saúde que relataram altos níveis de imobilidade tônica, uma resposta peritraumática involuntária e reflexa evocada em situações de ameaça intensa e inescapável, exibiram aumento na probabilidade de um provável diagnóstico de TEPT. Por outro lado, reconhecimento profissional teve relação negativa com sintomas de TEPT e depressão, emergindo como importante fator de proteção para a saúde mental. Identificar fatores protetores ou de risco nessas situações é fundamental para orientar a adoção de medidas de longo prazo nos ambientes de trabalho que melhorem a saúde mental desses profissionais.

Abstract The presence of unsaturated flow in tropical lateritic soils of unknown hydromechanical behavior may lead to severe geotechnical problems. It is known that soil-water characteristic curves (SWCC) are a valuable tool to define this behavior and facilitate the suction estimation, acting on tests performed with no control of such parameter. This article aimed to determine and model the SWCC for 3 unprecedented tropical soils, in the undisturbed and the compacted conditions, from the Paraná state, located in the south of Brazil. The soils studied are one clay and two sands, all lateritic. The pressure plate and the filter paper methods were used to determine the SWCC. The samples were carved and submitted to the drying paths. Furthermore, Mercury Intrusion Porosimetry (MIP) tests were performed in samples of the studied soils in order to obtain the pore size distribution (PSD), contributing to the bimodal definition of curves and the prediction of SWCC main parameters. Both techniques (pressure plate and filter paper) were combined and the SWCC was adjusted by Gitirana & Fredlund model, which efficiently represents the shape of the traditional curves for tropical soils. Furthermore, the SWCCs were predicted by PSD and obtained satisfactory numerical fits. All SWCC presented air entry values in the macropores that are characteristic of soils as the lateritic, given that its structure was considered a key factor in the shape of the curves.

Abstract Objective: To evaluate the periapical healing following root canal treatment in teeth with apical periodontitis (in vivo) and the cytotoxic potential of root canal sealers in vitro. Material and Methods: Apical periodontitis was induced in 60 dogs' teeth and root canals were filled with Sealapex (40 roots), EndoREZ (40 roots), intracanal dressing (20 roots), or left untreated (20 roots). After 30 and 90 days, histopathological analyses were made. In vitro, J774.1 macrophages were stimulated with root canal sealers extracts, cytotoxicity was assessed using lactate dehydrogenase assay, and qRT-PCR was used to analyze TNF-α gene expression. Results: In vivo, smaller apical periodontitis and lower inflammatory cell infiltrate were found in teeth treated with Sealapex compared to EndoREZ. In vitro, EndoREZ was cytotoxic and induced TNF-α gene expression by macrophages differently from Sealapex. Conclusion: Sealapex allowed improved tissue repair following root canal treatment in teeth with apical periodontitis compared to EndoREZ. Synthesis of TNF-α induced by LPS was enhanced by EndoREZ, whereas Sealapex prevented pro-inflammatory gene expression.

Abstract Background The relationship between Multidrug Resistant-Gram Negative Bacteria (MDR-GNB) infection and colonization in critically ill COVID-19 patients has been observed, however, it is still poorly understood. This study evaluated the risk factors for acquiring MDR-GNB in patients with severe COVID-19 in Intensive Care Units (ICU). Methods This is a nested case-control study in a cohort of 400 adult patients (≥ 18 years old) with COVID-19, hospitalized in the ICU of 4 hospitals in the city of Curitiba, Brazil. Cases were critical COVID-19 patients with one or more MDR GNB from any surveillance and/or clinical cultures were taken during their ICU stay. Controls were patients from the same units with negative cultures for MDR-GNB. Bivariate and multivariate analyses were done. Results Sixty-seven cases and 143 controls were included. Independent risk factors for MDR bacteria were: male gender (OR = 2.6; 95% CI 1.28‒5.33; p = 0.008); the hospital of admission (OR = 3.24; 95% CI 1.39‒7.57; p = 0.006); mechanical ventilation (OR = 25.7; 95% CI 7.26‒91; p < 0.0001); and desaturation on admission (OR = 2.6; 95% CI 1.27‒5.74; p = 0.009). Conclusions Male gender, desaturation, mechanical ventilation, and the hospital of admission were the independent factors associated with MDR-GNB in patients in the ICU with COVID-19. The only modifiable factor was the hospital of admission, where a newly opened hospital posed a higher risk. Therefore, coordinated actions toward a better quality of care for critically ill COVID-19 patients are essential.

Abstract Purpose: To evaluate and compare the effects of homologous and heterologous PRP (Platelet-Rich Plasma) on the quality and speed of skin wound healing, compared to Poor Platelet Plasma (PPP). Methods: Twenty-one male adult rabbits were used; two for preparing homologous PRP, with the rest of them separated randomly in three groups, according to the treatment received: PPP - control (n=5), homologous PRP (n=7), heterologous (n=7). Excisional skin wounds were made on the back of the animals, for the application of homologous and heterologous PPP and PRP. At the 14th post-operative day (POD), the animals were subjected to a new wound, and the treatments were inverted. The wounds were evaluated macroscopically and histologically. Results: A larger percentage of scar retraction was observed on the group treated with heterologous PRP, compared to homologous PRP, at the third POD, an increase of 25.03% (p=0.01). No other statistically significant differences among treatments were observed. Among every group, skin healing was efficient, without local adverse effects. Conclusions: Heterologous PRP contributed with more tissue retraction at the beginning of the wound healing process. After this, there were no differences on the wound healing skin process treated with PRP or PPP. However, our findings suggest the presence of others plasmatic factors, besides platelets, which could also contribute to the wound healing process, and thus, should be further investigated.

Resumo O objetivo desse estudo foi caracterizar a morfologia polínica das espécies de Grewioideae ocorrentes no estado do Rio de Janeiro. Grãos de pólen de 12 espécies foram examinados: tribo Apeibeae (Apeiba tibourbou, Corchorus hirsutus, Triumfetta althaeoides, T. grandiflora, T. obscura, T. rhomboidea) e tribo Grewieae (Luehea candicans, L. conwentzii, L. divaricata, L. grandiflora, L. ochrophylla, L. paniculata). Os grãos de pólen foram acetolisados, medidos, descritos e ilustrados sob microscopia de luz (ML). Para observar detalhes da superfície e abertura, grãos de pólen não acetolisados foram analisados em microscópio eletrônico de varredura (MEV) e, posteriormente, eletromicrografados. Foram caracterizados os grãos de pólen quanto à forma, ao tamanho, ao tipo de abertura, à polaridade e à constituição da exina. As espécies analisadas possuem grãos de pólen médios a grandes, isopolares, peroblatos a prolatos, 3-cólporos e sexina reticulada. Na chave polínica, a maioria das espécies pôde ser separada pelos atributos palinológicos. Isso permite considerar que as espécies aqui estudadas são euripolinícas, uma vez que é possível reconhecer a maioria das espécies pelas características palinológicas. Acredita-se que, o presente estudo fornece dados polínicos que podem auxiliar na identificação das espécies de Grewioideae que ocorrem no estado do Rio de Janeiro.

Abstract The objective of this study was to characterize the pollen morphology of the Grewioideae species occurring in the state of Rio de Janeiro. Pollen grains of 12 species were examined: tribe Apeibeae (Apeiba tibourbou, Corchorus hirsutus, Triumfetta althaeoides, T. grandiflora, T. obscura, T. rhomboidea) and tribe Grewieae (Luehea candicans, L. conwentzii, L. divaricata, L. grandiflora, L. ochrophylla, L. paniculata). The pollen grains were acetolysed, measured, described and illustrated under light microscopy (LM). To observe the surface and opening, pollen grains not acetolysed were analyzed in scanning electron microscope (SEM) and subsequently eletromicrographs. Were characterized pollen grains as the shape, size, opening type, the polarity and the exina constitution. The analyzed species have pollen grains medium to large, isopolar, peroblate to prolate, 3-colporate and reticulated sexine. In pollen key, most species could be separated those attributes palynological. It allows consider that species which were studied here are euripolinic, since it is possible to recognize most species by palynological characteristics. It is believed that the present study provides pollen characteristics data that may help identify the species of Grewioideae that occur in the State of Rio de Janeiro.

Abstract INTRODUCTION: Aedes aegypti and Culex quinquefasciatus are vector species responsible for the transmission of important arboviruses. METHODS: Adult mosquitoes were collected in the urban areas of four municipalities in Mato Grosso within 1 year. RESULTS: A total of 19,110 mosquitoes were collected. Among them, 16,578 (86,8%) were C. quinquefasciatus (44% female and 56% male); 2,483 (13%), A. (Stegomyia) aegypti (54% female and 46% male); and 49 (0,30%), from the genus Psorophora, Anopheles, Coquilettidia, and Sabethes. A significant correlation was observed between the number of mosquitoes from all species and dew point (female mosquitoes, p = 0.001; male mosquitoes, p = 0.001). CONCLUSIONS: The results of this study may be used as environmental indicators of mosquito populations.

RESUMO O objetivo deste trabalho foi avaliar a influência dos fatores ambientais sobre o desempenho das progénies de diferentes touros da raça Nelore no semiárido brasileiro. Informações de 741 animais foram coletadas de uma fazenda comercial localizada no municipio de Varzelândia-MG. Os animais eram filhos de 27 touros da raça Nelore e eram categorizados como LA (livro aberto) ou PO (puros de origem). Foram avaliados os dados de peso, ganho de peso médio diário e perímetro escrotal. As avaliações foram realizadas aos 12 (ano) e 18 (sobreano) meses de idade. Os dados foram analisados pelo método dos quadrados mínimos, utilizando um modelo que considerou efeito de categoria (LA ou PO), touro, anos, mês de nascimento e sexo dos bezerros. Houve influência significativa da categoria, sexo, mês de nascimento e touro sobre o desempenho dos animais até o ano, enquanto as características de desempenho ao sobreano foram influenciadas somente pelo mês de nascimento e touro (P<0,05). Sendo assim, os fatores do meio como ano e mês de nascimento e sexo do bezerro são importantes fontes de variação sobre o desempenho dos bezerros do nascimento até o sobreano, devendo, portanto, ser consideradas na comparação e seleção de animais com base nestas características.

SUMMARY The aim of this study was to evaluate the influence of environmental factors on the performance of progeny from different Nellore sires in Brazilian semiarid region. Data on live weight, average daily gain and scrotal circumference of 741 animals categorized as OR (open registry) or PB (purebred) and born from 27 sires were collected from a commercial farm located in Varzelândia-MG. Evaluations were taken at 12 (yearling) and 18months of age. Data were analyzed using the least-squares method, considering the effect of pedigree (OR or PB), sire, year of birth, month of birth and calf sex in the model. There was a significant influence of the pedigree, sex, month of birth and sire on animal's performance until yearling age, whereas the performance traits at 18months were influenced only by the month of birth and sire (P<0.05). Therefore, environmental factors such as year, month of birth and calf sex are important sources of variation on calf performance from birth to18 months and it should be considered when comparing and selecting animals based on these traits.

Resumo Objetivo: descrever a organização dos serviços de saúde para diagnóstico e tratamento dos casos de tuberculose na cidade de Manaus, Amazonas, Brasil. Métodos: estudo descritivo de 156 casos novos de tuberculose diagnosticados e residentes em Manaus, em 2014, caracterizados quanto aos aspectos socioeconômicos, demográficos, processo saúde-doença, relação com os serviços de saúde e resultado do tratamento, mediante aplicação de questionários individuais no 1o e no 6o mês de tratamento. Resultados: cerca de 70% dos casos foram diagnosticados no centro de referência para tuberculose; a maioria deles desconhecia existir agente comunitário de saúde em seu bairro; quanto ao acompanhamento de tratamento, 55,3% necessitam ingressar em filas para marcação de consultas; somente 67,3% dos casos foram acompanhados pelo mesmo profissional durante todo o tratamento. Conclusão: os achados indicam presença de barreiras no diagnóstico e tratamento dos casos, tanto no centro de referência como nas unidades de atenção primária à saúde.

Abstract Objective: to describe the organization of health services for tuberculosis case diagnosis and treatment in the city of Manaus, Amazonas, Brazil. Methods: this was a descriptive study of 156 new tuberculosis cases diagnosed and residing in Manaus in 2014, characterized according to socioeconomic, demographic, health-disease process aspects, relationship with health services and treatment outcome, by administering individual questionnaires in the 1st and 6st months of treatment. Results: some 70% of cases were diagnosed at the tuberculosis reference center; most of the cases were unaware that there was a community health agent in their neighborhood; regarding treatment follow-up, 55.3% had to join an appointment waiting list; only 67.3% of cases were monitored by the same professional throughout entire treatment. Conclusion: the findings indicate presence of barriers to case diagnosis and treatment, both in the referral center and also in primary healthcare units.

Resumen Objetivo: describir la organización de los servicios de salud para el diagnóstico y tratamiento de la tuberculosis en Manaus, Amazonas, Brasil. Métodos: estudio descriptivo de 156 casos nuevos de tuberculosis diagnosticados y residentes en Manaus en 2014, caracterizados cuanto a aspectos socioeconómicos, demográficos, proceso salud-enfermedad, relación con los servicios de salud y resultado del tratamiento, mediante la aplicación de cuestionarios individuales en el primero y el sexto mes de tratamiento. Resultados: aproximadamente 70% de los casos fueron diagnosticados en el centro de referencia para tuberculosis; la mayoría desconocía la existencia de un agente comunitario de salud del barrio; en cuanto al seguimiento de tratamiento, 55,3% necesitan obtener fichas para marcar las consultas; sólo un 67,3% de los casos fueron acompañados por el mismo profesional durante todo el tratamiento. Conclusión: los hallazgos indican la presencia de barreras para el diagnóstico y el tratamiento de casos, sea en el centro de referencia o en unidades de atención primaria de salud.

Resumo Objetivo: descrever a organização dos serviços de saúde para diagnóstico e tratamento dos casos de tuberculose na cidade de Manaus, Amazonas, Brasil. Métodos: estudo descritivo de 156 casos novos de tuberculose diagnosticados e residentes em Manaus, em 2014, caracterizados quanto aos aspectos socioeconômicos, demográficos, processo saúde-doença, relação com os serviços de saúde e resultado do tratamento, mediante aplicação de questionários individuais no 1o e no 6o mês de tratamento. Resultados: cerca de 70% dos casos foram diagnosticados no centro de referência para tuberculose; a maioria deles desconhecia existir agente comunitário de saúde em seu bairro; quanto ao acompanhamento de tratamento, 55,3% necessitam ingressar em filas para marcação de consultas; somente 67,3% dos casos foram acompanhados pelo mesmo profissional durante todo o tratamento. Conclusão: os achados indicam presença de barreiras no diagnóstico e tratamento dos casos, tanto no centro de referência como nas unidades de atenção primária à saúde.

Abstract Objective: to describe the organization of health services for tuberculosis case diagnosis and treatment in the city of Manaus, Amazonas, Brazil. Methods: this was a descriptive study of 156 new tuberculosis cases diagnosed and residing in Manaus in 2014, characterized according to socioeconomic, demographic, health-disease process aspects, relationship with health services and treatment outcome, by administering individual questionnaires in the 1st and 6st months of treatment. Results: some 70% of cases were diagnosed at the tuberculosis reference center; most of the cases were unaware that there was a community health agent in their neighborhood; regarding treatment follow-up, 55.3% had to join an appointment waiting list; only 67.3% of cases were monitored by the same professional throughout entire treatment. Conclusion: the findings indicate presence of barriers to case diagnosis and treatment, both in the referral center and also in primary healthcare units.

Resumen Objetivo: describir la organización de los servicios de salud para el diagnóstico y tratamiento de la tuberculosis en Manaus, Amazonas, Brasil. Métodos: estudio descriptivo de 156 casos nuevos de tuberculosis diagnosticados y residentes en Manaus en 2014, caracterizados cuanto a aspectos socioeconómicos, demográficos, proceso salud-enfermedad, relación con los servicios de salud y resultado del tratamiento, mediante la aplicación de cuestionarios individuales en el primero y el sexto mes de tratamiento. Resultados: aproximadamente 70% de los casos fueron diagnosticados en el centro de referencia para tuberculosis; la mayoría desconocía la existencia de un agente comunitario de salud del barrio; en cuanto al seguimiento de tratamiento, 55,3% necesitan obtener fichas para marcar las consultas; sólo un 67,3% de los casos fueron acompañados por el mismo profesional durante todo el tratamiento. Conclusión: los hallazgos indican la presencia de barreras para el diagnóstico y el tratamiento de casos, sea en el centro de referencia o en unidades de atención primaria de salud.

RESUMO O objetivo deste estudo é descrever os resultados de um Consenso Brasileiro sobre Neuropatia de Fibras Finas (NFF). Quinze neurologistas (membros da Academia Brasileira de Neurologia) revisaram uma versão preliminar do artigo. Onze panelistas se reuniram na cidade de Fortaleza para discutir e terminar o texto para a submissão do manuscrito. NFF pode ser definida como um subtipo de neuropatia caracterizada pelo envolvimento seletivo de fibras sensitivas amielínicas ou pouco mielinizadas. Seu quadro clínico inclui manifestações negativas e positivas: sensitivas (dor/disestesias/prurido) ou queixas sensitivas e autonômicas combinadas, associadas a exame neurológico quase totalmente normal. A eletromiografia convencional é normal. Uma lista crescente de condições médicas causa NFF. NFF também pode servir como uma terminologia útil para referenciar pequenas discrepâncias nos valores normais de diferentes laboratórios de neurofisiologia. Diferentes técnicas podem evidenciar anormalidades sensitivas e/ou autonômicas. São necessários mais estudos para refiná-las e para o desenvolvimento de terapias específicas.

ABSTRACT The aim of this study was to describe the results of a Brazilian Consensus on Small Fiber Neuropathy (SFN). Fifteen neurologists (members of the Brazilian Academy of Neurology) reviewed a preliminary draft. Eleven panelists got together in the city of Fortaleza to discuss and finish the text for the manuscript submission. Small fiber neuropathy can be defined as a subtype of neuropathy characterized by selective involvement of unmyelinated or thinly myelinated sensory fibers. Its clinical picture includes both negative and positive manifestations: sensory (pain/dysesthesias/pruritus) or combined sensory and autonomic complaints, associated with an almost entirely normal neurological examination. Standard electromyography is normal. A growing list of medical conditions is associated with SFN. The classification of SFN may also serve as a useful terminology to uncover minor discrepancies in the normal values from different neurophysiology laboratories. Several techniques may disclose sensory and/or autonomic impairment. Further studies are necessary to refine these techniques and develop specific therapies.

Resumo Objetivo: descrever a distribuição geográfica dos hospedeiros intermediários do Schistosoma mansoni em cinco estados brasileiros. Métodos: estudo transversal; foram selecionados municípios dos estados do Paraná (78), Minas Gerais (120), Bahia (82), Pernambuco (51) e Rio Grande do Norte (98), nos anos de 2012 a 2014; esses municípios foram escolhidos por não possuírem registros atualizados da presença de caramujos hospedeiros intermediários de S. mansoni; moluscos foram capturados, taxonomicamente identificados e examinados para verificação de cercárias de S. mansoni. Resultados: os trabalhos foram realizados em 427 municípios (99,5% dos 429 selecionados); foi registrada presença de moluscos em 300 (70,2%) municípios e a ocorrência de Biomphalaria glabrata em 62 (21%) municípios, B. straminea em 181 (60%), B. tenagophila em três (1%); associação de B. glabrata/B. straminea foi encontrada em 53 (18%), e de B. glabrata/B. tenagophila em um (0,3%). Conclusão: os registros de B. glabrata, B. straminea e B. tenagophila estão de acordo com a distribuição conhecida.

Abstract Objective: to describe the geographical distribution of intermediate hosts of Schistosoma mansoni in five Brazilian states. Methods: this was a descriptive cross-sectional study; municipalities were selected in the states of Paraná (78), Minas Gerais (120), Bahia (82), Pernambuco (51) , and Rio Grande do Norte (98), for the period 2012 to 2014; these municipalities were chosen because they did not have current records of the presence of snails vectores de S. mansoni. The molluscs were captured and taxonomically identified and examined for S. mansoni cercariae. Results: the work was carried out in 427 municipalities (99.5% of the 429 selected); the presence of mollusks was registered in 300 (70.2%) municipalities; Biomphalaria glabrata were found in 62 (21%) municipalities, B. straminea in 181 (60%), B. tenagophila in three (1%); B. glabrata/B. straminea association was found in 53 municipalities (18%) and B. glabrata/B. tenagophila association in one (0.3%) municipality. Conclusion: B. glabrata, B. straminea and B. tenagophila distribution records obtained in this study are consistent with previously known distribution.

Resumen Objetivo: describir la distribución geográfica de los hospedadores intermediarios de Schistosoma mansoni en cinco estados brasileños. Métodos: estudio epidemiológico transversal; el estudio fue realizado en municipios de los estados de Paraná (78), Minas Gerais (120), Bahia (82), Pernambuco (51) y Rio Grande do Norte (98), entre 2012 y 2014; estos municipios fueron elegidos por no poseer registros actualizados de la presencia de caracoles vectores de S. mansoni; los moluscos fueron capturados, taxonómicamente identificados y examinados para la verificación de cercarias de S. mansoni. Resultados: los trabajos fueron realizados en 427 municipios (99,5% de 429 municipios seleccionados); fue registrada presencia de moluscos en 300 (70,2%) municípios; la presencia de Biomphalaria glabrata fue registrada en 62 (21%) municipios, B. straminea en 181 (60%) y B. tenagophila en três (1%); se observó asociación de B. glabrata con B. straminea en 53 (18%) y de B. glabrata con B. tenagophila en uno (0,3%). Conclusión: los registros de Biomphalaria están de acuerdo con la distribución conocida.

Resumo Objetivo: descrever a distribuição geográfica dos hospedeiros intermediários do Schistosoma mansoni em cinco estados brasileiros. Métodos: estudo transversal; foram selecionados municípios dos estados do Paraná (78), Minas Gerais (120), Bahia (82), Pernambuco (51) e Rio Grande do Norte (98), nos anos de 2012 a 2014; esses municípios foram escolhidos por não possuírem registros atualizados da presença de caramujos hospedeiros intermediários de S. mansoni; moluscos foram capturados, taxonomicamente identificados e examinados para verificação de cercárias de S. mansoni. Resultados: os trabalhos foram realizados em 427 municípios (99,5% dos 429 selecionados); foi registrada presença de moluscos em 300 (70,2%) municípios e a ocorrência de Biomphalaria glabrata em 62 (21%) municípios, B. straminea em 181 (60%), B. tenagophila em três (1%); associação de B. glabrata/B. straminea foi encontrada em 53 (18%), e de B. glabrata/B. tenagophila em um (0,3%). Conclusão: os registros de B. glabrata, B. straminea e B. tenagophila estão de acordo com a distribuição conhecida.

Abstract Objective: to describe the geographical distribution of intermediate hosts of Schistosoma mansoni in five Brazilian states. Methods: this was a descriptive cross-sectional study; municipalities were selected in the states of Paraná (78), Minas Gerais (120), Bahia (82), Pernambuco (51) , and Rio Grande do Norte (98), for the period 2012 to 2014; these municipalities were chosen because they did not have current records of the presence of snails vectores de S. mansoni. The molluscs were captured and taxonomically identified and examined for S. mansoni cercariae. Results: the work was carried out in 427 municipalities (99.5% of the 429 selected); the presence of mollusks was registered in 300 (70.2%) municipalities; Biomphalaria glabrata were found in 62 (21%) municipalities, B. straminea in 181 (60%), B. tenagophila in three (1%); B. glabrata/B. straminea association was found in 53 municipalities (18%) and B. glabrata/B. tenagophila association in one (0.3%) municipality. Conclusion: B. glabrata, B. straminea and B. tenagophila distribution records obtained in this study are consistent with previously known distribution.

Resumen Objetivo: describir la distribución geográfica de los hospedadores intermediarios de Schistosoma mansoni en cinco estados brasileños. Métodos: estudio epidemiológico transversal; el estudio fue realizado en municipios de los estados de Paraná (78), Minas Gerais (120), Bahia (82), Pernambuco (51) y Rio Grande do Norte (98), entre 2012 y 2014; estos municipios fueron elegidos por no poseer registros actualizados de la presencia de caracoles vectores de S. mansoni; los moluscos fueron capturados, taxonómicamente identificados y examinados para la verificación de cercarias de S. mansoni. Resultados: los trabajos fueron realizados en 427 municipios (99,5% de 429 municipios seleccionados); fue registrada presencia de moluscos en 300 (70,2%) municípios; la presencia de Biomphalaria glabrata fue registrada en 62 (21%) municipios, B. straminea en 181 (60%) y B. tenagophila en três (1%); se observó asociación de B. glabrata con B. straminea en 53 (18%) y de B. glabrata con B. tenagophila en uno (0,3%). Conclusión: los registros de Biomphalaria están de acuerdo con la distribución conocida.